Expression of connective tissue growth factor and low density lipoprotein receptor related protein induced by transforming growth factor beta 1 in human pulmonary fibroblasts-1 / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-560140
ABSTRACT
Objective:
To investigate the tendency and correlation between connective tissue growth factor (CTGF) and low density lipoprotein receptor-related protein (LRP) in human pulmonary fibroblasts-1 (HPF-1) induced by transforming growth factor beta1 (TGF-?_1) at different times.Methods:
After the HPF-1 cells were stimulated with TGF-?_1 (5 ?g/L) at different times (0 h, 3 h,6 h,12 h and 24 h),CTGF and LRP mRNA expressions were analyzed by RT-PCR. The same preparation steps of cell culture were repeated, then, the protein expressions were determined by Western Blot, co-immunoprecipitation Western Blot and immunocytochemistry.Results:
The expressions of CTGF and LRP mRNA had similar tendency, and LRP (224.87?7.00) correlated with CTGF (131.53?2.86) positively (r= 0.8402, P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Peking University(Health Sciences)
Año:
2003
Tipo del documento:
Artículo
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