Protective effects and mechanisms of etomidate on anoxia injury in cultured rat hippocampal neurons / 中国药理学通报

ABSTRACT

Aim To investigate the protective effects of etomidate on cultured rat hippocampal neurons subjected to anoxia injury and its mechanism.Methods Hippocampal neurons of neonatal rat,which had been cultured in vitro for 10 days,were allocated to control groups and etomidate-treating groups.The neurons were exposed to oxygen-glucose deprivation for 24 h.The cell survival rate in each group was evaluated using MTT colorimetry.To explore the effect of etomidate on neuronal calcium overload evoked by anoxia or 50 mmol?L~(-1) KCl or 1 mmol?L~(-1) glutamate,fluo-3,a fluorescent probe,was used for imaging of intracellular calcium in laser scanning confocal microscope(LSCM)to measure real-time changes of [Ca~(2+)]_i in cultured rat hippocampal neurons.Results The hippocampal neurons developed acute neuronal swelling and widespread neuronal degeneration following anoxia for 24 h.Etomidate at concentrations of 1.2～4.8 mg?L~(-1) attenuated the neuronal injury in a dose-dependent manner(P