Effects of rapid eye movement sleep deprivation on cognitive function and expression of brain-derived neurotrophic factor in rat hippocampus / 第二军医大学学报

ABSTRACT

Objective:To investigate the effects of various degrees of rapid eye movement (REM) sleep deprivation (RSD) and sleep recovery on cognitive function (learning and memory) and expression of brain-derived neurotrophic factor (BDNF) in the rat hippocampus. Methods: Male SD rats were divided into 8 groups (n=12) blank control group (with normal sleep), environmental control, RSD 1 d, RSD 3 d, RSD 5 d, RSD 7 d, recover sleep 6 h after 7 d RSD (RS 6 h), and recover sleep 12 h after 7 d RSD (RS 12 h). The modified multiple platform method (MMPM) was used to establish sleep deprivation model in rats. The cognitive functions of rats were tested by Y-type maze. The hippocampal BDNF mRNA and protein levels were detected by real-time PCR and immunohistochemical method. Results: The failure reaction times of all RSD groups and the 2 RS groups were significantly more than those in control group and environmental control group (P