Neuroprotective effect of acteoside against rotenone-induced damage of SH-SY5Y cells and its possible mechanism / 中国药理学通报

ABSTRACT

Aim To investigate the neuroprotective effect of acteoside against rotenone-induced cell damage in SH-SY5Y cells and the effect of acteoside on the expression of Parkinson disease (PD)-related proteins Parkin and ?-Synuclein(?-Syn), and to discover the underlying molecular mechanism of neuroprotection by acteoside. Methods The activity of lactate dehydrogenase(LDH) was measured by spectroscopy. Expressions of Parkin and ?-Syn were studied using Western blot analysis, and the distribution of ?-Syn was analyzed using immunofluorescence technique. Results ① Acteoside (10, 20 or 40 mg?L-1) pretreatment for 6 h markedly reduced the release of LDH induced by 0.5 ?mol ?L-1 rotenone; ② Treatment with 0.5 ?mol?L-1 rotenone for 48 h led to significant Parkin cleavage and increased formation of ?-Syn protein dimer; ③ Pretreatment of SH-SY5Y cells with acteoside (10, 20 or 40 mg?L-1) for 6 h markedly reduced the cleavage of Parkin induced by 0.5 ?mol?L-1 rotenone in a concentration-dependent manner, inhibited the aggregation of ?-Syn and decreased ?-Syn-positive SH-SY5Y cells. Conclusion These findings suggest that pretreatment of acteoside has a potent neuroprotective effect against rotenone-induced SH-SY5Y cells damage and its mechanism might involve in reducing the cleavage of Parkin and inhibitng ?-Syn expression induced by rotenone.