Expression of CD105 in oxygen-induced retinal neovascularization models in mice / 第二军医大学学报

ABSTRACT

Objective:To investigate the expression of CD105 in oxygen-induced retinopathy (OIR) in C57BL/6J mice.Methods: Forty 7-day old C57BL/6J mice were evenly randomized into normal control group and hyperbaric oxygen group. The OIR model was induced with hyperbaric oxygen; the retinal neovascularization was observed by H-E staining and the expression of CD105 in OIR was observed by immunohistochemistry staining. Results: H-E staining indicated that the retinal neovascularization model was successfully estabished. CD105 was strongly expressed in the hyperbaric oxygen group, with the integral photodensity of angiogenesis being 9 985.63?1 016.28 per mouse and the positive staining area being (14 246.61?6 052.29) ?m2 per mouse; CD105 was weakly expressed in the control group with the integral photodensity of angiogenesis being 1 625.36?638.44 per mouse and the positive staining area being (3 619.31?1 760.03) ?m2 per mouse; the differences between the 2 groups were statistically significant(P