Concentration of quinolinic acid in rat serum by GC-MS / 第三军医大学学报

ABSTRACT

Objective To establish a sensitive and accurate gas chromatography-mass spectrometry method for determination of quinolinic acid in rat serum.Methods The objective compound was purified and derivatized to its dihexafluoroisopropyl ester before quantitative determination by scanning method and quantitative determination by SIM method.Results The endogenous substances in serum had no interference on the measurement of quinolinic acid in the sample.Assay linearity was obtained in the range of 0.05-20 ?mol/L with limit of quantitation(LOQ)of 0.05 ?mol/L.The intra-day relative standard deviation(RSD)was lower than 15%.The inter-day relative standard deviation was lower than 12%.Conclusion It is a sensitive,accurate and convenient method.The dihexafluoroisopropyl ester of quinolinic acid is quite stable,but the serum should not be preserved for a long time before derivation.