Observation on cellular immune response in mice induced by co-immunization of Mtb8.4 gene vaccine and plasmid encoding human interleukin 12 / 解放军医学杂志

ABSTRACT

Objective To observe the specific cellular immune response induced by co-immunization of DNA vaccine of Mtb8.4 and plasmid encoding human interleukin 12(hIL-12)in mice.Methods Fifteen C57BL/6N mice were divided into following groupsMtb8.4 gene vaccine plus plasmid of hIL-12,Mtb8.4 gene vaccine,BCG,empty vector alone and PBS.Mice were immunized intramuscularly in both hind limbs three times at the intervals of three weeks or once subcutaneously with 1?106 of viable M.bovis BCG Pasteur at the time of the first DNA immunization.The level of IFN-? in supernatant of spleno-lymphocyte cultures was measured by ELISA.CTL activities of spleno-lymphocyte were detected with LDH release assay.Results The levels of IFN-? and IL-2 in supernatant of spleno-lymphocyte cultures in the group of Mtb8.4 gene vaccine plus plasmid of hIL-12 were significantly higher than that of group of Mtb8.4 alone(P