Determination of Serum Tryptophan and Kynurenine in Pregnant Women / 浙江中医药大学学报
Article
en Zh
| WPRIM
| ID: wpr-561149
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WPRO
ABSTRACT
[Objective] To establish a method for determination of serum tryptophan and kynurenine in pregnant women.[Method] RP-HPLC was applied to analysis.C18 column was used.The mobile phase was composed of methanol-0.001M KH2PO4(PH=4)(12∶88).The UV detection wave length was 245nm.[Results] The linear response ranged from 2.56~307.06?mol?L-1 for tryptophan, r=0.9999.The linear response ranged from 0.12~5.05?mol?L-1 for kynurenine, r=0.9997.The mean concentrations of serum tryptophan and kynurenine were 49.73?7.48?mol?L-1 and 0.74?0.33?mol?L-1 respectively in 30 pregnant women.[Conclusion] The method is sensitive and suitable for the determination.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Zhejiang Chinese Medical University
Año:
2006
Tipo del documento:
Article