Silence the Expression of Bcl-2 Gene in Cisplatin-Resistant Cell Line Acc-3/Cddp / 医学研究杂志
Journal of Medical Research
;
(12)2006.
Artículo
en Chino
| WPRIM
| ID: wpr-561445
ABSTRACT
Objective To investigate the interfering efficiency of RNAi technique on the expression of Bcl-2 oncogene in human oral adenoid cystic carcinoma (Acc-3/CDDP).Methods The recombined RNAi plasmids for Bcl-2 oncogene were constituted by the four successive steps-designing of Oligo DNAs, synthesis of Oligo DNAs, transfection of Oligo DNAs into pSUPER.neo+gfp vectors and selection of positive plasmids. In order to silence the expression of Bcl-2 oncogenes, the recombined RNAi plasmids were transfected into Acc-3/CDDP cells by culturing together for about 10 hours, and the interfering efficiency of RNAi for the two oncogenes was evaluated by fluorescence-quantitative RT-PCR.Results The interfering efficiencies for Bcl-2 oncogene were 0,66.20% and 0, respectively in psiB1、siB2、psiB3.Conclusions The recombined RNAi plasmids of psiB2 (CCgggAgATAgTgATgAA) for Bcl-2 oncogene can effectively silence the expression of Bcl-2 oncogenes in Acc-3/CDDP cell line.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Medical Research
Año:
2006
Tipo del documento:
Artículo
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