Your browser doesn't support javascript.
loading
Detect PEDF mRNA by RT-PCR in Experimental Choroidal Neovascularization Induced by Krypton Laser / 医学研究杂志
Journal of Medical Research ; (12)2006.
Artículo en Chino | WPRIM | ID: wpr-561572
ABSTRACT
Objective This study was designed to establish an experimental Choroidal neovascularization (CNV) model in BN rats, which could exhibit high reproducibility and to evaluate the expression of pigment epithelium-derived factor (PEDF). Methods 30 rats were received krypton laser. 1 days, 3 days, 7 days, 14days, 21 days and 28 days after photocoagulation, fundus fluorescein angiography (FFA) wAS performed. Total RNA were abstracted from retinal and choroidal tissue and the mRNA of PEDF was detected by RT-PCR.Results FFA showed flurescein leakage from 1 week to 4 weeks after photocoagulation. The mRNA of PEDF were in significantly difference during 4 weeks by RT-PCR.Conclusions Kryton laser photocoagulation can be successfully used to produced CNV experimental model in the BN rat. This study gave us a effective way to evaluate the mechanism of CNV by RT-PCR.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio pronóstico Idioma: Chino Revista: Journal of Medical Research Año: 2006 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio pronóstico Idioma: Chino Revista: Journal of Medical Research Año: 2006 Tipo del documento: Artículo