Effect of Tongluo Jiunao Oral Solution on Learning and Memory and the Expression of AChE in Hippocampus of AD Rats / 中华中医药杂志

ABSTRACT

Objective: To observe the effect of Tongluo Jiunao oral solution on learning and memory and the expression of AChE in hippocampus of AD rats induced by ?-amyloid1-40.Methods: 140 SD rats were divided into blank control group(normal group),pseudo-operation control group,negtive control group(model group),positive control group and experiment group(48、24、12mg?kg-1?d-1)at random.The model of AD were established by injecting ?-amyloid1-40 into bilateral hippocampus.After 4 weeks,Morris Water Maze was used to determine the ability of learning and memory.Immunohistochemical method and image analysis techniques were used to determine the expression of AChE in Hippocampus.Results: Compared with normal group,in place navigation test,the mean escape latencies of the model group increased obviously,and in spatial probe test,the frequency of passing through the platform of the model group decreased obviously(P