Effects of advanced glycated bovine serum albumin on tubule like structure formation of endothelial cells and their mechanisms / 第三军医大学学报

ABSTRACT

Objective To observe the effects of advanced glycated bovine serum albumin(AGE-BSA) on the tubule like structure(TLS) formation of endothelial cells.Methods There were three groupsAGE-BSA(25,50 and 100 ?g/ml) treated groups,BSA(25,50 and 100 ?g/ml) treated groups as BSA control groups,endothelial complete medium without AGE-BSA or BSA as blank control groups.After incubation of human umbilical vein endothelial cells(HUVECs) for 24,48 and 72 h,TLS formation assay and ELISA were used to detect the length of TLS formation on the surface of collagen Ⅰ and the vascular endothelial growth factor(VEGF) autocrine of HUVECs in presence of AGE-BSA.Results In 24 to 72 h,AGE-BSA(25,50,100 ?g/ml) increased the average length of TLS formation on the surface of collagen Ⅰ and the concentration of VEGF autocrine in supernatant of HUVECs(P