Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
en Zh
| WPRIM
| ID: wpr-562151
Biblioteca responsable:
WPRO
ABSTRACT
Objective To study the effects of monocyte chemotactic protein-1(MCP-1) on the growth of human umbilical vein endothelial cells (hUVEC). Methods hUVEC were cultured in vitro and identified. hUVEC that grew vigorously were stimulated for 24 h or 48 h respectively with MCP-1(0.1, 1.0, 10, and 100 ?g/L). The survival rate of hUVEC was first detected by MTT assay. The cell cycle and DNA content were detected and analyzed by flow cytometry. Results hUVEC were isolated from human umbilical veins and cultured, and then identified by immunofluorescence and immunohistochemistry with factor Ⅷ and KDR. MCP-1 induced the apoptosis of hUVEC in a dose-dependentmanner and a time-dependentmanner (P
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Índice:
WPRIM
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Journal of Third Military Medical University
Año:
2003
Tipo del documento:
Article