The role of tumor necrosis factor related apoptosis inducing ligand and Polypeptide from Chlamys farreri in UVA-induced apoptosis in HaCaT cells / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-562335
ABSTRACT
Aim To investigate the impact of UVA on expression of tumour necrosis factor related apoptosis inducing ligand (TRAIL) and study the role of TRAIL in UVA-induced apoptosis of HaCaT cells as well as the influence of Polypeptide from Chlamys farreri(PCF)on TRAIL apoptotic pathway induced by UVA.Methods Cells were divided into five groups control group, UVA model group,UVA+5.69 mmol?L-1 PCF group, UVA+2.84 mmol?L-1 PCF group, UVA+1.42 mmol?L-1 PCF group.Expression level of TRAIL mRNA was assayed by Real-Time PCR.Western blot analysis was used to determine the protein level of TRAIL and caspase-8 activation. The effect of TRAIL neutralization antibody on UVA-induced apoptosis was also investigated.Results TRAIL mRNA and protein levels increased after 8 J?cm-2 UVA radiation and the discrepancy was significant compared with control group(P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Pharmacological Bulletin
Año:
2003
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS