Distribution and clinical significance of human papillomavirus subtypes in Shenzhen People's Hospital / 第三军医大学学报

ABSTRACT

Objective To investigate the distribution of human papillomavirus(HPV) subtypes in condyloma acuminatum(CA) patients in Shenzhen People's Hospital and assess the association between HPV subtypes and cervix neoplasm.Methods Type specific prevalence and extent of multiple infection were assessed in the genital tract CA samples collected from 352 patients visiting Departments of dermatology or gynecology of Shenzhen People's Hospital during 2003-2004.PCR using MY09/11 as primer and reverse dot blot hybridization for the genotyping of 9-20 HPV subtypes.Results HPV type diversity was broad in the investigated CA patients.The low risk HPV type(HPV subtype 11,6) was dominant and multiple HPV infections occurred in 37% of HPV-positive samples.High risk HPV type was dominant in CA from cervix,especially in the high grade cervical intraepithelial neoplasia(CIN Ⅱ+),and multiple high risk HPV(HR-HPV) infection was found in 87% of HPV positive samples.Conclusion HPV subtypes 6 and 11 were dominant in the patients visiting Shenzhen People's Hospital.HPV subtypes 16 and 18 may be the main causes of malignant changes of cervix.