Inhibitive effect of specific stealth siRNAs on TGF-?_1 expression of mouse lung fibroblasts / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-562466
ABSTRACT
Objective To design and produce specific stealth siRNAs and detect their inhibitive effects on TGF-?1 expression.Methods Three stealth siRNAs aimed directly at different sequences(stealth-48,stealth-166,stealth-594) in TGF-?1 mRNA were made.These siRNAs were transfected into BALB/c mouse lung fibroblast in vitro,then the TGF-?1 expression in those lung fibroblasts was detected by immunohistochemistry.Results In different time periods,the TGF-?1 expression was differently depressed by three stealth siRNAs in vitro.The inhibitory effects of stealth-166 was better than the other two stealth siRNAs.The inhibition could be detected in 48 h,reached the highest level in 72 h and began to attenuate 96 h later.Conclusion The TGF-?1 expression of mouse lung fibroblasts in vitro can be depressed by synthetic stealth siRNAs.It seems possible to provide a new way for the treatment of pulmonary interstitial fibrosis.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Third Military Medical University
Año:
2003
Tipo del documento:
Artículo
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