Effects of thrombospondin-1 typeⅠ repeat antisense RNA on human endothelial cells / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12)1987.
Artículo
en Chino
| WPRIM
| ID: wpr-562883
ABSTRACT
Aim To investigate the effects of thrombospondin-1 type Ⅰ repeat antisense RNA on cell proliferation and growth activity of human endothelial cells.Methods The eukaryotic expression vector of pcDNA3.1-/anti-TSP-1-Ⅰ was constructed.Then the vector was transfected into human umbilical vein endothelial cells(HUVECs).The growth activity of endothelial cells after transfection was detected by MTT.The variation of endothelial cell's cycle was determined by flow cytometry.Cell morphological change was observed by transmission electron microscope.Results The eukaryotic expression vector(pcDNA3.1-/anti-TSP-1-Ⅰ)was successfully constructed and transfected into HUVECs.The OD value of pcDNA3.1-/anti-TSP-1-Ⅰ transfective group was promoted compared with that of nontransfective group(P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Pharmacological Bulletin
Año:
1987
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS