Establishment of mouse model of premature ovarian failure / 第三军医大学学报

ABSTRACT

Objective To develop a mouse model of premature ovarian failure (POF) by chemical treatment or radiotreatment. Methods Eighty Kunming mice were divided into four groups, including chemical treatment group that were administered Cyclophosphamide and Busulfan, radiotreatment group receiving 60Co ? ray irradiation, control group and castrated group. To evaluate the animal model, vaginal smears were collected daily for estrous cycle determination, and serum hormone levels and histological changes in ovaries were examined. Results Estrous cycles became irregular after chemical treatment or radiotreatment (P0.05). Compared to control group, ovaries from chemical treatment and radiotreatment groups showed ovarian atrophy and interstitial fibrosis. The number of growing follicles and non-atretic primordial follicles was notably reduced (P