The Expression and Function of Fca Receptor on Human Airway Smooth Muscle Cells / 医学研究杂志

ABSTRACT

Objective To investigate the expression and function of Fca receptor on human airway smooth muscle(ASM)cells.Methods RT-PCR and immunofluorescence technique were used to detect the expression of Fca receptor on human ASM cells.The effect of IgA on intracellular calcium concentration of human ASM cells was measured by using Fura-2/AM as a calcium indicator.Results RT-PCR and immunofluorescence staining confirmed the presence of Fca receptor on human ASM cells.Compared with control,secretory IgA(sIgA)induced a rise in intracellular calcium concentrations on human ASM cells after 90min incubation(P0.05).Conclusion Fca receptor was expressed on human ASM cells.SIgA increased the intracellular calcium concentration of human ASM cells.