Relationship of local recurrence with expression of protein P53 and PCNA in laryngeal carcinoma and its surgical margins / 第二军医大学学报

ABSTRACT

Objective:To explore the relationship of the local recurrence with the expression of protein P53 and PCNA in the primary lesions and the surgical margins of laryngeal carcinoma.Methods:The primary lesions and the surgical margins of la- ryngeal carcinoma of 36 patients were made into serial sections.Immunochemical method(H-E staining)was used to detect the expression of P53 and PCNA protein.Results.The positive rates of protein P53 and PCNA were 55.6%(20/36)and 88.9% (32/36)in the primary lesions,and 25%(9/36)and 30.6 %(11/36)in the surgical margins,respectively.The recurrent rates of the primary lesions positive for P53 and PCNA were 35%(7/20)and 25%(8/32),respectively,which were higher than those negative for them(12.5% [2/16]and 25% [1/4]).The recurrent rates of surgical margins positive for p53 and PCNA were 44.4%(4/9)and 72.7%(8/11),respectively,which were higher than those negative for them(18.5% [5/27]and 4%[1/ 25]).The expression of P53 and PCNA proteins in the primary lesions and surgical margins was abnormal;the recurrent rate of the laryngeal carcinoma was 100%(4/4)after surgery.Conclusion:P53 and PCNA protein can be used as biomarkers for local recurrence of laryngeal carcinoma after operation.Detection of P53 and PCNA in both the primary lesions and the surgical mar- gins may be of greater significance in forecasting local recurrence.