Effect and mechanism of Anandamide on the proliferation of human lung cancer A549 cell line / 中国药理学通报

ABSTRACT

Aim The effect of N-arachidonoyl ethanolamide(Anandamide,AEA),on the human lung cancer cell A549 was analysed the possible mechanism was detected.Methods To assess the sensitivity of A549 to AEA,A549 cells were exposed to increasing doses of AEA with or without the antagonists to vanilloid receptor 1(VR1) and aspirin.MTT methods were employed to investigate A549 cell proliferation.Results A549 cells exhibited dose-dependent sensitivity to AEA resulting in dramatic cell death.But the effect of AEA on A549 could not be antagonized by the antagonists such as capsazepine and Ruthenium Red.However,cyclooxygenase(COX) inhibitor,aspirin,could attenuate A549 cell death caused by AEA(P