Troglitazone inhibits proliferation of GH3 cell in vitro / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2001.
Artículo
en Chino
| WPRIM
| ID: wpr-564211
ABSTRACT
Objective:
To study the anti-proliferation effects of thiazolidinedione compounds-troglitazone, which is a high affinity ligand of PPAR-? , on rat pituitary adenoma GH3 cell line and explore the related mechanisms.Methods:
GH3 cells were separately treated with troglitazone (10-7,10-6 and 10-5 mol/L), dimethyl sulfoxide (DMSO) (DMSO control group) and phenol red- and serum-free F-12 medium (blank group). MTT was used to examine the cell growth in each group and FACS was used to detect the distribution of cell cycle. Semi-quantitative RT-PCR method was utilized to determine the expression of CyclinD1 mRNA. ANOVA was used for statistical analysis.Results:
The 72 h treatment with troglitazone inhibited GH3 cell proliferation in a dose-Dependent manner. The treatment also induced cell cycle arrest in G1/S phase and significantly decreased the expression of CyclinD1 mRNA as compared to the other 2 groups (P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Academic Journal of Second Military Medical University
Año:
2001
Tipo del documento:
Artículo
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