Growth inhibition effects of artesunate on human cervical cancer cell line HeLa / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
en Zh
| WPRIM
| ID: wpr-564242
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effects of artesunate on human cervical cancer cell line HeLa.Methods The inhibitory effect on cell proliferation was assessed by MTT assay.Transmission electron microscopy and fluorescent staining were applied to demonstrate the presence of apoptosis.Cell cycle,reactive oxygen species(ROS) levels and mitochondrial membrane potential(??m) were examined by flow cytometry,respectively.Expression of caspase-3 was detected by immunohistochemical methods.Results Growth inhibition of ART on HeLa cells was time-and dose-dependent.Apoptotic feature was observed by transmission electron microscopy and fluorescent staining.The blocking of the cell cycle was in S-phase.The expression of caspase-3 in cytoplasm was positive.The generation of ROS increased obviously(P
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Zh
Revista:
Journal of Third Military Medical University
Año:
2003
Tipo del documento:
Article