Effect of goat autologous serum at different concentrations on proliferation of bone marrow-derived mesenchymal stem cells / 第三军医大学学报

ABSTRACT

Objective To investigate the effect of goat autologous serum at different concentrations on the proliferation of bone marrow-derived mesenchymal stem cells(bMSCs) for optimal serum dose.Methods Autologous serum was prepared from 6 goats at 12 months old,and their bMSCs were isolated from bone marrow.Then the obtain bMSCs were cultured in DMEM/F12 medium with supplement of 5%,10%,or 15% of corresponding autologous serum,and 10% fetal bovine serum served as control.The morphology of bMSCs,cells counting and MTT assay were observed or carried out.Results Morphology of the bMSCs in the different groups were similar.The proliferation of 5% autologous serum group was slower compared with that of 10% fetal bovine serum group(P