Effects of ?-interferon on proliferation and B7 expression in SPC-A-1 cell line of human lung adenocarcinoma / 中国实用内科杂志
Chinese Journal of Practical Internal Medicine
; (12)2006.
Article
en Zh
| WPRIM
| ID: wpr-564753
Biblioteca responsable:
WPRO
ABSTRACT
Objective To study the effects of IFN-?on expression of B7 molecules in human lung adenocarcinoma cell line cultured in vitro.Methods Human lung adenocarcinoma SPC-A-1 cells were incubated in the medium with IFN-?respectively.Cell proliferation effect was measured by MTT assay;apoptosis was detected with flow cytometry assay;expression of B7-1 and B7-2 mRNA was determined by RT-PCR.Results Compared with those of control group,MTT shows that IFN-?could reduce the SPC-A-1 cell proliferation.FCM shows the apoptosis rate in the IFN-? group was significantly higher,but there is no different changes in each concentration group.Compared with the control group,expression of B7-1 and B7-2 significantly increased in the IFN-?group,and the expression was not correlated with the concentration of IFN-?in each control group.Conclusion IFN-?can inhibit cell proliferation and induce apoptosis in SPC-A-1 cell line.IFN-?markedly enhance the expression of B7-1 and B7-2 in SPC-A-1 line.The apoptosis may be mediated by up-expression of gene B7-1 and B7-2.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Practical Internal Medicine
Año:
2006
Tipo del documento:
Article