Study on Determination of Tannins in Rosa laecigata Michx. by Casein Method / 中华中医药杂志

ABSTRACT

To establish a stable method for determining the tannins in Rosa laevigata Michx. by casein method. The optimum conditions of determination were adopting 400mg caseins,adding 1.5% Na2CO3 and detemining after 30 min. The selected method has a good liner dependance. The average recovery of tannin acid was 100.5%,RSD=1.85%. The method is rapid and reliable for the determination of tannins in Rosa laevigata Michx. and determined the contents of the fruits in eight different disturbs and xi’an 's content has the highest,3.96%.