DETERMINATION OF POLYUNSATURATED FATTY ACID IN BREAST MILK BY HPLC / 营养学报

ABSTRACT

Objective To develop HPLC method for determination of polyunsaturated fatty acid(PUFA) in breast milk.Method PUFA were extracted from breast milk with mixture of methanol N-hexanephosphoric acid(15253).PUFA were derived by phenacyl bromide.The mobile phase was acetonitrilewater(8119,v/v) at 1.0 ml/min with UV detection at 252 nm and column temperature was 35℃.The analytic column was Hypersil BDS C18(4.6mm?150mm,5 ?m).Results The average recovery was(98?5)% and the relative standard deviation of intra-day and inter-day were limited within 5.9%.Conclusion The method was stable,sensitive and accurate,and suitable for determination of PUFA in breast milk.