Detection of Sample Preparation about Sialy-LewisA/X on the Surface of Colon Carcinoma Cells by Flow Cytometry / 医学研究杂志

ABSTRACT

Objective To investigate the effects of variable sample preparation about sialy LewisA/X on the surface of colon carcinoma cells.Methods Human colon carcinoma cell suspension was prepared by using EDTA,trypsin,cell scraper,trypsin-EDTA and trypsin-cell scraper.Cell counts and sialy-LewisA/X on the surface of colon carcinoma cells were detected by flow cytometry.Results Trypsin made fluorescence intensity of sialy-LewisA/X on the surface of colon carcinoma cells become weaken.Fluorescence intensity of sialy-LewisA/X had no significant deviation among EDTA,cell scraper and trypsin-cell scraper.Cell counts were multitude in EDTA group or trypsin-cell scraper group.Conclusion Trypsin-cell scraper is a available approach on preparing cell suspension of adherent cell.