Effects of volatile isoflurane on rat heart ischemia-reperfusion injury in vitro / 中国药理学通报

ABSTRACT

Aim To investigate the effects of Isofluraneon function,metabolism,ATPase activity and free radicals in isolated ischemia /reperfusion(I/R) rat hearts.Methods 56 SD rats were randomly divided into 7 small groups(n=7),or 2 big groups according to given drug.In a normal thermal isolated Langendorff rat heart model,Myocardial Adenosine triphosphate(ATP),Malodialdehyde(MDA),Activity of Ca2+-ATPase,Na+,K+-ATPase and Superoxide Dismutase(SOD)were determined.Results In isoflurane group,LVDP and dp/dt decreased,CF and ATP increased while the activity of Na+,K+-ATPase remained unchanged.The myocardial ATP content decreased slowly in isoflurane group.At the end of reperfusion,ventricular function,CF,myocardial ATP content and the activity of Ca2+-ATPase and SOD in isoflurane group were significantly higher while myocardial MDA was significantly lower than those in control group.Conclusion Isoflurane can depress myocardial systolic function and activity of Ca+-ATPase in normal myocardium,improve the recovery of function and metabolism,and increase CF and the activity of Ca2+-ATPase and Na+,K+-ATPase.