Comparison of Quality of Peripheral Blood Stem Cell in Healthy Donors and Hematologic Malignancies Patients with rhG-CSF Mobilization / 医学研究杂志

ABSTRACT

Objective To investigate the blood cell separator collection of peripheral blood stem cell after using recombinant human granulocyte colony - stimulating factor (rhG - CSF) to mobilize the normal donor and patients with hematologic malignancies, through morphological observation of lymphocyte ratios and PBSC vitality of mononuclear cells. Methods Peripheral blood mononuclear cells (MNC)were collected from 15 cases of healthy donors and 14 patients of hematologic malignancies with rhG - CSF mobilization dose with blood cell separators. The number of nucleated cells, classification of nucleated cells, MNC count and cell viability were observed. Results ①The total number of nucleated cells in normal donor group was(4.31?1.41)?10~8 / kg,and hematologic malignancies group was (6.21?4.37)?10~8/kg. ②The number of nucleated cells of PBSC in hematologic malignancies group was higher than those of the donor group (P0.05).④MNC number was (96.7? 5.1) % in normal donor group counts, and in which lymphocytes was (65.9?9.4)% , myeloid (16.3?8.7)% , mononuclear cells (16.5?4.0)% ,respectively,MNC number was (92.7?15.6)% in hematologic malignancies group, and in which lymphocytes was (55.3?16.7)% , myeloid (24.3?16.5) % , mononuclear cells (14.9?9.1)%, respectively. ⑤Cell viability in normal donor group before and after cryopreservation were (91.5?4.3)%, (67.4?9.1) % , in hematologic malignancies grou were (82.9?11.1) % , (56.5?20.1) % , respectively. Cell viability was significantly different (P