Effect of metformin on the inhibinB,activinA and follistatin in polycystic ovary model rats / 中国药理学通报

ABSTRACT

Aim To observe the effect of metformin(MF)on inhibinB,activinA and follistatin in rat of polycystic ovary(PCO),analyzing the possible therapeutic mechanism and therapeutical effect from ovaries local cytokine.Methods The PCO rat models were set by insulin combined with human chorionic gonadotrophin(HCG).The levels of serum gonadal hormone and insulin(INS)were detected by radioimmunoassay.Using the sandwich ELISA technique the expressed level of serum inhibinB(INHB),activinA(ACTA)and follistatin(FS)was detected.Results Metformin could decrease the level of testosterone(T),luteotropic hormone(LH)and INS in rat serum,but it increased the level of ACTA in rat serum and the rate of ACTA/INHB.There was negative correlation between INS and ACTA,and positive correlation between INS and INHB.Conclusion MF can regulate the expression of ovaries local cytokines such as INHB,ACTA and FS.