Influence of a mimic 7-amino acid peptide of HCV HVR1 on cytokines secretion of immune cells / 解放军医学杂志

ABSTRACT

Objective To demonstrate the frequency changes in immune cells on secreting interleukin-10 (IL-10),interferon-? (IFN-?),tumor necrosis factor-? (TNF-?) and interleukin-4 (IL-4) under the stimulation of a mimic 7-amino acid peptide of HCV HVR1 (7P),and to explore the mechanism of such changes. Methods The peripheral blood mononuclear cells (PBMCs) were isolated from healthy people and co-cultured with 7P. Intracellular cytokine staining was performed using antibodies against IL-10,IFN-?,TNF-? and IL-4. The related cells were harvested on a FACSCalibur flow cytometer,and then analyzed with FACSCalibur software. Results Compared to the controls,the frequency of immune cells secreting IFN-? was significantly increased in both CD4+CD8-,CD4-CD8+ T cells and NK,NKT cells (P