Expression of TGF-?1 and CTGF and Intervention of Leflunomide in Rats with Diabetes / 医学研究杂志

ABSTRACT

Objective To study the expression of TGF-?1 and CTGF and the prevention effects of leflunomide in diabetic nephropathy rats,and to study the mechanism of curing DN of leflunomide.Methods Thirty-six male Wistar rats were randomly divided into three groups : control group,diabetic model group,diabetes treated with leflunomide group.After being removed the right renal of rats,diabetic model was created by injecting STZ(40mg/kg),while control group received identical volum of citrate buffer solution.When the model was established,rats in the leflunomide-treated group were daily garvage of leflunomide(5mg/kg).The same capacity saline was given to control group and diabetic model group.24-hour urine volume and 24-hour urine protein were determined after eight and twelve weeks,as well as serum creatinine and urea nitrogen.The kidney sections were studied for pathological changes with light microscopy.The expression of TGF-?1 and CTGF was determined by immunohistochemistry respectively.Results With the course extending,immunohistochemistry analysis showed that the expression of TGF-?1 and CTGF increased significantly in DN group,as compared with control group,while TGF-?1 and CTGF reduced in diabetes treated with leflunomide group(P