Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus
Journal of Veterinary Science
;
: 175-180, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-56722
ABSTRACT
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Infestaciones por Garrapatas
/
Glicoproteínas
/
Immunoblotting
/
Enfermedades de los Bovinos
/
Cromatografía de Afinidad
/
Secuencia de Aminoácidos
/
Análisis de Secuencia de Proteína
/
Ixodidae
/
Electroforesis en Gel de Poliacrilamida
/
Focalización Isoeléctrica
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2007
Tipo del documento:
Artículo
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