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Activation of adenosine receptor A2a up-regulates epithelial sodium channel ?-subunit in cultured alveolar epithelium cells / 第三军医大学学报
Journal of Third Military Medical University ; (24)2002.
Artículo en Chino | WPRIM | ID: wpr-567844
ABSTRACT
Objective To investigate the effects of adenosine receptor A2a on the expression of epithelial sodium channel ?-subunit (?-ENaC) in alveolar epithelium A549 cells and the effects of adenosine receptor A2a in acute lung injury/aute respiratory distress syndrome. Methods After alveolar epithelium A549 Cells were incubated with 0,0.1,1,10 and 100 ?mol/L adenosine receptor A2a agonist CGS-21680 for 8 h or with 100 ?mol/L CGS-21680 for 0,1,4,8,24 and 48 h respectively,the mRNA and protein levels of ?-ENaC were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis respectively. Results After A549 cells were incubated with CGS-21680 at different doses for 8 h,the mRNA and protein levels of ?-ENaC were elevated significantly at 0.1 ?mol/L CGS-21680 treatment compared with the control group (P

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Journal of Third Military Medical University Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Journal of Third Military Medical University Año: 2002 Tipo del documento: Artículo