MiR-203 inhibits proliferation and invasion of esophageal squamous cell carcinoma / 第二军医大学学报

ABSTRACT

Objective To investigate the influence of miR-203 on the proliferation and invasion of human esophageal squamous cell carcinoma cell line Eca109.Methods Double-stranded mimics of miR-203 were designed and transfected into Eca109 cells with Lipofectamine 2000;Eca109 cells transfected with nonsense microRNA mimics were taken as control.The proliferation ability of Eca109 cells was determined by calculating the cell population doubling time and the percentage of apoptotic cells;the invasion ability of Eca109 cells was determined by Transwell assay.Results In vitro experiment showed that,compared with the control group,Eca109 cells transfected with miR-203 mimics had a significantly longer cell population doubling time([26.1?0.5]h vs [24.2?0.6]h,P