IMMUNOHISTOCHEMICAL STUDY OF A- AND B-CELLS OF RATS' PANCREATIC ISLETS DURING EXPERIMENTAL PEPTIC ULCER / 解剖学报

ABSTRACT

Forty rats were selected for the experiment, which were divided into three groups including experimental ulcer group, normal saline and normal control groups. The pancreatic tissue were prepared for immunohistochemical staining at 6th, 10th, 14th, and 21st day after operation. Sternberger's PAP method was used to show the glucagon cell (A cell) and insulin cell (B cell) in order to observe their changes during the experimental peptic ulcer. In normal control group, the percentage of A cells is 23.31?1.91 (X?SD) and that of B cells is 73.1?4.01. But in experimental ulcer group, the A cell percentage is raised up to 42.6?5.59 and the B cell percentage is lowered down to 57.5?4.55 on the 10th day after operation. Comparing with normal saline group and normal group their differences show statistical outstanding differences. The result may point out that both A and B cells of pancreatic islet take part in the body's natural resistance against the disease, especially A cells.