A HIGH EFFICIENT METHOD FOR THE SEPARATION OF CELL FRACTION ENRICHED WITH INTERMEDIATE AND LATE ERYTHROBLAST CELLS / 解剖学报

ABSTRACT

A method described by Harrison has been adopted and modified by us for the separation of intermediate and late erythroblast cells from 15 day embryonic liver of pregnant Wistar rat. The method consisted briefly of preparation of fetal liver cell suspension and the separation of cell types in a 40% and 70% nonlinear Percoll gradient system. Using this method, we can obtain about 96% of hemogenous population of intact and viable intermediate and late erythroblasts. Examination of tho separated cells by Giemsa and benzidine staining and by electron microscopic observation indicated that no granulocytes or other white blood cells could be detected, except for some contamination of about 1% of proerythroblasts and 3% of reticulocytes in the fraction. Trypan blue vital staining demonstrated that there were over 95％ of the cells maintained viable after separation, they could be used directly for the study of cell differentiation as well as biochemical analysis. SO, this is an economical, simple and easy technique to operate which proved to be a useful mean for obtaining enrich population of intermediate and late erythroblasts in research in the field of cell biology.