STEREOLOGIC ANALYSIS OF THE LYMPHO CYTES LOCATED AT INTRA-AND EXTRAFOLLICULAR DENDRITIC CELL DISTRIBUTION AREA / 解剖学报

ABSTRACT

HRP-anti-HRP complex was injected into BALB/c mice through the tail vein. 24 hours later, the spleens of mice were removed, fixed and cut into slices. After incubation in DAB/H_2O_2 the follicular dendritic cell (FDC) distribution area could be defined at a certain part of lymph nodule. Light microscopic and stereologic analysis study on the lymphocytes at intra- and extra- FDC distribution area were carried out. The results revealed that lymphocytes at intra- and extra- FDC distribution area were composed of various lymphocytes populations of different sizes. The cell populations at intra-FDC distribution area were mainly composed of large and medium sized lymphocytes, while the populations at extra-FDC distribution area were mainly medium sized and small lymphocytes. As a result, FDC always exists on the location where lymphoblastic phenomenon appears. This indicates that FDC has certain relation with the lymphoblastic phenomenon.