Determination of Fraxetin A and Fraxetin B in Cortex Fraxini by HPLC / 中成药

ABSTRACT

The determination of fraxetin A and fraxetin B in Cortex Fraxini have been carried out by HPLC on the stationary phaseoctodecyl-silicohydride bonded silica gel column. The mobile phase was methanol-water(2476). The detection wavelength was at 348nm. There was a good linear relationship in a concentration range of 16?g/ml~176?g/ml. The correlation coefficients were 0. 9995 and 0. 9994,respectively. The recovery of the added sample were 99. 1 ?1. 5% (n=5) for fraxetin A and 99.8?2. 5%(n=5) for fraxetin B. This method showed 0. 8% of within-day accuracy and 6. 2% of between-day accuracy,involving the advantages of simplicity,quickness and accuracy.