Reaserch of relationship between Wnt/?-Catenin signal path-way in gastric carcinoma and the expression of Caspase-3 and Bcl-2 induced in vitro by indomethici / 重庆医科大学学报

ABSTRACT

Objective:To study the regulatory mechanism of Wnt/?-Catenin passageway influenced apoptosis of human gastric cancer line SGC-7901 by detecting the expression of?-Catenin mRNA,Caspase-3 mRNA and bcl-2 mRNA.Methods:(1) Methyl thiazolyl tetrazolium(MTT)assay was used to measure the anticancer activity of indomethaci.(2)Expression of?-Catenin, Caspase-3 mRNA and bcl-2 mRNA in groups of without induced or induced by indomethaci were detected by RT-PCR. Results:Indomethacin of 50?mol/L at 24h couldn't induce apoptosis of gastric cancer cells.Indomethacin of 50?mol/L at 48 and 72h and of 100?mol/L and 200?mol/L at 24,48 and 72h could induce apoptosis of gastric cancer cells in adose- and time-dependent manner;Compared with groups of without induced by indomethaci,expression of?-Catenin mRNA and bcl-2 mRNA were lower,however,increased expression of Caspase-3 mRNA were found in SGC7901 cells after treatment with indomethaci.Conclusion:Apoptosisin inbalance may be caused by activating expression of bcl-2 proto-oncogene protein and inhibiting expression of Caspase-3 protein through Wnt/?-Catenin passageway in human gastric cancer line SGC7901.