Content Determination of Total Flavones in Runchang Ningshen Extract / 广州中医药大学学报

ABSTRACT

To establish a method for the determination of the content of total flavones and the quality control of Runchang Ningshen Extract. [Methods & Results]With rutin as control,the optimal concentration of Runchang Ningshen Extract was obtained by refluxing for three times with 70% ethanol.Ultraviolet spectrophotometry was used.The regression equation was induced at detecting wavelength being 511nm and with sodium nitrite and aluminium nitrate as color developing agent.There was a good linearity (r=0 9999)in the range of 8 ?g.The average recovery of total flavones was 94 63%(RSD=2 58%,n=5).[Conclusion]This method is simple and reproducible and can serve as the standard for controlling the quality of Runchang Ningshen Extract.