Analysis of amygdalin of Ephedra spp.and Apricot Kernel Cough Lozenge by HPLC / 中成药

ABSTRACT

Objective: Assay of amygdalin of Ephedra spp. and Apricot Kernel Cough Lozenge (ephedrine hydrochloride, Semen Armeniacae Amarum, etc.) by HPLC. Methods: Amygdalin was extracted with methanol. The separation was performed by HPLC and amygdalin was determined with UV detector. Results: The contents of amygdalin of Ephedra spp. and Apricot Kernel Cough Lozenge was 0.476% and 1.53mg per lozenge. Conclusion: The method was simple, accurate, rapid and with good reproducibility. It can be used for quality control of Ephedra spp. and Apricot Kernel Cough Lozenge.