Applications of RHD zygosity test through polymerase chain reaction for prediction of fetus Rh D-positive phenotype / 中华妇产科杂志

ABSTRACT

Objective To explore a molecular method for the determination of RHD zygosity. Methods Two pairs of primers were designed specific for downstream rhesus box and hybrid rhesus box according to the sequences in GenBank. Together with a pair of internal control primer, a dual-tube polymerase chain reaction (PCR) method was established for determination of the RHD zygostity. Thirty Rh D-positive, D-negative and weak D phenotype samples were evaluated by taking a recent restriction fragment length polymorphism (RFLP) method as reference. Results The results of the dual-tube PCR and RFLP are identical in 29 samples, which are also in concordance with the Rh phenotype, respectively. In one weak D sample, however, both methods tested as RHD -/RHD -, which showed that there was one RHD - haplotype in this individual, but a false negative result in testing downstream rhesus box. Conclusions The dual-tube PCR is a less-labored method for RHD zygosity detection. It can be used clinically in prenatal test to determine RHD zygosity of pregnant women′s partner for prediction of fetus RhD phenotype.