The expression of P-gp during the MDR1 gene transferring into the hematopoietic cells of the Balb/c mouse bone marrow / 重庆医科大学学报

ABSTRACT

Objective:The study was to improve the protection effect of the bone marrow during the chemotherapy of earlier hepatocarcinoma Balb/C mice,to observe the expression of P-gp in tumor tissues and important organs and to examine the influence of MDR1 gene on Balb/C mouse. Methods:MDR1 gene was transferred into hematopoietic cells of murine bone marrow by the mediation of retrovirus vecter and transplanted into the Balb/c mouse with hepatocarcinoma exposure with 60 Co-? ray in advance.The function and the expression of MDR1 gene in hematopoietic cells,and tumor tissue was detected by RT-PCR method and IC method in vitro.Results:There was no P-gp expression in the two control groups,but that of the different dose experiment group was higher than that of control groups obviously ( P