Identification of Rhizoma Alismatis by HPLC Combined with Evaporation Light Scattering Determination Method / 中药新药与临床药理
Article
en Zh
| WPRIM
| ID: wpr-571309
Biblioteca responsable:
WPRO
ABSTRACT
Objective To establish a method for the identification of Rhizoma Alismatis. Methods HPLC combined with evaporation light scattering detector(ELSD) was applied. Chromatographic column was Macherey-Nagel C18(250 mm?4 mm,10 ?m),acetonitrile -water (4 ∶1) used as mobile phase,flow rate 0.5 mL/min,gas flow rate of ELSD 2.25 L/min and the temperature of drift tube at 70 ℃. Results In the extraction of the authentic Rhizoma Alismatis,both alisol A24-acetate and alisol B23-acetate could be estimated;in the extraction of Rhizoma Alismatis from the market,the alisol A24-acetate could be estimated only;and only alisol B23-acetate could be estimated in the extraction of the standard powder of Rhizoma Alismatis. Conclusion The method is efficient,practical and easy to operate for detecting and identifying Rhizoma Alismatis.
Texto completo:
1
Índice:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Traditional Chinese Drug Research & Clinical Pharmacology
Año:
1993
Tipo del documento:
Article