Determination of tetrahydropalmatine in Juyanbaokang Granules by SPE-HPLC / 中成药

ABSTRACT

Objective: To determine tetrahydropalmatine in Juyanbaokang Granules (Rhizoma Corydalis, Radix Aconcti Lateralis Preparata, Radix Rauwolfiae, etc.) by SPE-HPLC. Methods: Samples were purified by C 18 SPE column. HPLC column was Dikma Diamonsil C 18 (250mm?4.6mm,5?m), mobile phase was 0.05M potassium dihydrogen phosphate-0.005M sodium heptanesulfonate-acetonitrile (1.2∶1.2∶1), flow rate was 1.2 mL?min -1 and detection wavelength was 230nm. Results: The calibration curve was linear in the range of 0.067 ～0.402?g for tetrahydropalmatine ( r =0.9997). The average recovery was 98.66%. Conclusion: The method with good separation is convenient, rapid, accurate and reliable.