Effect of ultraviolet C irradiation on expression of basic fib roblast growth factor of wound in rats / 中华物理医学与康复杂志

ABSTRACT

Objective To investigate the influence o f ultraviolet C (UVC) irradiation of different doses on expression of basic fibrob last growth factor (bFGF) in granulation tissues of wound in rats. MethodsThirty Wistar rats were recruited and three wounds wer e made in each rat. The UVC irradiation of different doses (60mJ/cm2 and 15mJ/ cm2) were performed daily in two of the three wounds in each rat, respectively , and the UVC irradiation lasted for three days. The expression of bFGF both at the mRNA level and the protein level were observed by the methods of in situ hyb ridization and immunohistochemistry at 7 days, 14 days, 21 days after wound was made, respectively. ResultsAt the 7 days after woun d was made, the expression of bFGF in irradiation groups were higher than that i n the control group ( P