Application of single nucleotide polymorphism in the study of the susceptible gene of microangiopathy in diabetes mellitus / 重庆医科大学学报

ABSTRACT

Objective:To detect the distribution of the (C-106T) single nucleotide polymorphism and genotype of promoter region of aldose reductase gene and to study its relation to the susceptibility of microangiopathy in type 2 diabetes mellitus in Chinese Han from Chongqing.Methods:The aldose reductase gene in promoter region was amplified by polymerase chain reaction and the frequency of genotype of type 2 diabetes were analyzed by restriction enzyme BfaI.Results:The frequencies of genotype CC were significantly higher and the frequencies of genotype TT were much lower in type 2 diabetes patients with microangiopathy than in those without microangiopathy (? 2=6.78 and 4.45,respectively,P