Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Jieduwanling Pills by HPLC / 中成药

ABSTRACT

AIM: To establish the method for determining ephedrine hydrochloride and pseudoephedrine hydrochloride in Jieduwanling Pills (Rhizoma atractylodis,Herba ephedrae,Herba dendrobii,etc.)by RP-HPLC. METHODS: The Agilent Extend C_ 18 column with acetonitrile-0.1%H_3PO_4(5∶95)as the mobile phase was used. The flow rate was at 1.0 mL?min -1 and the detective wavelength was at 206 nm. RESULTS: The calibration curve was linear at a range of 0.02028 ～1.014?g for the ephedrine hydrochloride( r =0.9999). the average recovery was 97.69% and RSD was 0.9%( n =6). The calibration curve was linear at a range of 0.02254 ～ 1.127 ?g for the pseudoephedrine hydrochloride( r =0.9998). The average recovery was 98.40% and RSD was 2.3%( n =6). CONCLUSION: The method is accurate with a good reproducibility and can be used as a quantitative analysis for this preparation.